Saliva Cortisol Test
What is Cortisol and Why Does it Matter ?
Cortisol is often called the “stress hormone” because it’s produced by the adrenal glands in response to both physical and mental stress. However, cortisol does more than just manage stress—it also plays an essential role in:
- Regulating Metabolism: Cortisol helps balance blood sugar levels and influences how your body uses proteins, fats, and carbohydrates.
- Controlling Inflammation: It helps modulate immune responses, which can affect everything from allergies to autoimmune disorders.
- Supporting Energy Levels: Cortisol naturally peaks in the morning, helping you wake up, and typically tapers off at night to prepare you for rest.
When cortisol levels become imbalanced—either too high or too low—symptoms can include fatigue, insomnia, mood swings, weight changes, and a weakened immune system.
Why Test Cortisol in Saliva ?
1. Concentration Levels
Blood cortisol levels are often up to 100 times higher than salivary cortisol. This is because saliva contains only a small fraction of the hormone compared to total cortisol in the bloodstream.
2. Free Cortisol vs. Total Cortisol
- Saliva Measures Free Cortisol: Salivary cortisol reflects the unbound (or “free”) hormone that’s immediately active in the body.
- Blood Measures Total Cortisol: Most cortisol in the bloodstream is attached to proteins, making it less readily available to act on tissues.
3. Non-Invasive Collection
Collecting blood can be stressful and potentially skew cortisol results. In contrast, saliva collection is simple, painless, and can be done at home—reducing stress-related fluctuations.
4. Correlation with Stress Levels
Salivary cortisol is thought to correlate more closely with daily stress and free blood cortisol levels, offering a more accurate snapshot of how your body responds to stressors in real time.
Common Reasons to Measure Cortisol
- Chronic Fatigue or Burnout: If you feel tired all the time, understanding cortisol fluctuations can help identify underlying adrenal fatigue or other issues.
- Screening for Cushing Syndrome: Salivary cortisol testing is recognized as a useful, non-invasive screening tool for conditions like Cushing syndrome.
- Diagnosis of Cortisol Imbalances: Saliva tests help identify both cortisol excess and deficiency, as well as monitor hydrocortisone replacement therapy.
- Sleep Disturbances: Abnormal cortisol rhythms can contribute to insomnia, restlessness, or poor sleep quality.
- Mood & Anxiety Issues: High or erratic cortisol levels can exacerbate stress, anxiety, and irritability.
- Imbalanced Lifestyle: Excessive exercise, poor diet, and lack of rest can disrupt cortisol production over time.
- Long-Term Health Monitoring: Conditions like Cushing’s syndrome (excess cortisol) or Addison’s disease (low cortisol) benefit from frequent cortisol tracking.
How it Works
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Order Your Test
Place your order online, and schedule Home Visit. -
Collect Samples
Follow our detailed instructions to collect saliva at specific times (e.g., early morning, noon, late afternoon, and evening). This helps capture the natural ebb and flow of your cortisol levels. We will collect the samples and process this in the lab. -
Receive Your Results
Within days, you’ll receive a detailed report outlining your cortisol levels at each collection point.
Who Should Consider This Test ?
This test is suitable for anyone who suspects chronic stress, experiences persistent fatigue or anxiety, or wants a proactive approach to health. It’s also beneficial if you’re juggling a hectic lifestyle, managing multiple responsibilities, or noticing changes in your sleep or energy patterns
The Importance of 24 Hour Saliva Cortisol Test
Cortisol naturally follows a daily rhythm, rising to its highest levels in the early morning and gradually tapering off toward bedtime. By collecting saliva samples at specific times
- waking (around 7–8 a.m.) - Captures the morning cortisol surge
- midday (around 12 p.m.) – Checks mid-range levels.
- late afternoon (around 4 p.m.) – Assesses afternoon dip.
- before bedtime (around 10–11 p.m.) – Evaluates evening drop, just before sleep.
a 24-hour saliva cortisol test can reveal abnormalities such as adrenal fatigue, chronic stress, or hormonal imbalances. This comprehensive snapshot helps healthcare providers better understand how well your body manages stress throughout the entire day.
What is the role of Cortisol in Longevity?
Saliva cortisol provides a window into the body’s stress response, which has a direct impact on healthspan and longevity. Over time, persistently high or dysregulated cortisol can contribute to chronic inflammation, metabolic disturbances, and impaired immune function—all of which are associated with age-related diseases.
By measuring cortisol through non-invasive saliva tests, individuals can identify abnormal daily patterns (e.g., elevated evening levels or a flattened diurnal curve) that may speed up the aging process.
Tracking and managing cortisol levels—through stress reduction techniques, proper sleep, nutrition, and if necessary, medical intervention—helps maintain a healthier hormonal balance, potentially supporting both a longer lifespan and a higher quality of life.
What are the Test Guidelines ?
It is recommended to avoid eating, drinking (other than water), or brushing your teeth for atleast 30 minutes before each collection.
About the Quality
Quality assurance is done at 3 levels
- Sample collection from home using one time sealed QR coded sterile kits and transportation to the lab including the temperature condition requirements
- Sample acceptance and QC at the lab
- Sample run at the NABL/CAP/ISO certified labs
How Accurate Are Saliva Cortisol Test?
When performed and interpreted correctly, saliva cortisol tests are a reliable method for assessing active cortisol levels. Most reputable labs use standardized protocols and quality controls to ensure accuracy. However, always follow the collection instructions carefully and consult with a qualified healthcare professional to interpret results in the context of your overall health.
What Can the Test tell me about My Health ?
By measuring cortisol at multiple time points, the test reveals whether your cortisol follows a normal daily rhythm. It can detect patterns that indicate high or low cortisol levels, suggesting issues like chronic stress, adrenal fatigue, or other hormonal imbalances. This information helps healthcare providers create personalized plans for stress management, lifestyle changes, or further medical evaluation
How Does a 24-Hour Saliva Cortisol Test Differ From a Single Sample Test ?
A single sample only provides a snapshot, while multiple samples across a full day capture the normal diurnal rhythm—peaking in the morning and decreasing by night. This 24-hour pattern is crucial for diagnosing issues like adrenal fatigue or abnormal cortisol spikes in the evening
- For those who want to identify risk factors of chronic conditions before it develops as a disease.
- To evaluate key body parameters and avoid the risk to major organs of your body.
- No fasting is required.
- Please do not consume alcohol 24-48 hours before the test.
- Avoid eating, drinking (other than water), or brushing your teeth for atleast 30 minutes before each collection.